Fig. 2.

The role of MLK7-AS1 in regulating ovarian cancer cell proliferation, colony formation, and apoptosis. (a) Comparison of interference efficiency of three MLK7-AS1 small interfering RNA sequences. (b) Cell growth viability was assayed in SKOV3, OVCAR3, and PEO1 cells transfected with si-NC, si-MLK7-AS1–1 or − 2 using MTT at 0 h, 24 h, 48 h, 72 h and 96 h time point. (c) Knockdown of MLK7-AS1 suppressed colony formation in SKOV3, OVCAR3, and PEO1 cells. (d) Cell apoptosis analysis was performed using flow cytometry. (e) Apoptosis related markers: Bcl-2, Bax, Bak and cleaved caspase 3 were detected using western blot assay in SKOV3, OVCAR3, and PEO1 cells transfected with si-NC, si-MLK7-AS1–1 or − 2. Data presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01