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. 2018 Jun 20;12(10):2458–2469. doi: 10.1038/s41396-018-0205-y

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© International Society for Microbial Ecology 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Proteolytic activity and gene expression of Rhl-dependent reporters in various genotypes and conditions. a Proteolytic activity was measured using an elastin-congo red assay for each of the genotypes (methods). b GFP expression average of single cells as measured using a P_lasB-GFP reporter during growth in casein medium. Results are shown for three genotypes; ΔlasR (blue, overlapped by red), ΔlasR;psdR⁻ (red) and ΔlasR;psdR⁻;mexT⁻ (orange). Results were obtained using flow cytometry. c GFP per optical density as a function of time of GFP transcriptional reporters for lasB (top panel), rhlA (middle panel), and rhlI (bottom panel). Results in each panel are shown for four genotypes; mexT⁺ (light blue), ΔlasR (red), mexT⁻ (orange), and ΔlasR; mexT⁻(magenta). Error bars represent standard error of three or more independent repeats on different days. Exact genotypes are provided in Supplementary Tables S1, S2. *—p value < 0.05, **—p value < 2 × 10⁻³. (color figure online)