Table 2.
Genotyping of mexT and psdR of 13 blindly chosen isolates from a single evolved line of the ΔlasR strain
| Halo size phenotype | Number of isolates | psdR allele | Effect | mexT allele | Effect | CmR |
|---|---|---|---|---|---|---|
| Ancestral | 4 | Wild-type | Wild-type | + | ||
| Ancestral | 1 | C209T | Q → Stop | Wild-type | + | |
| Ancestral | 1 | A529C | T → P | Wild-type | + | |
| Ancestral | 1 | A389C | V → A | Wild-type | + | |
| Evolved | 4 | R431C | Y → S | G502A | E → K | − |
| Evolved | 1 | R431C | Y → S | A176C | D → A | − |
| Evolved | 1 | R431C | Y → S | Δ (51-165) | Truncation | − |
The first column indicates the halo size on skim-milk; Ancestral (small) or evolved (large). The last column indicates whether the strains can grow in 100 μg/mL of chloramphenicol