Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 21;8:14228. doi: 10.1038/s41598-018-32591-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Biochemical characterization, far-UV CD spectra, and thermal stability of cALAS. (A) The yellow (left) and red (right) fractions after anion exchange chromatography of recombinant cALAS. (B) Thin-layer chromatography of the reaction products of the ALA formation assays with the purified wild-type and mutants of recombinant cALAS. ALA (lane 1) and glycine (lane 2) standards; reaction mixtures with the PLP form of the wild-type (lane 3), the heme form of the wild-type (lane 4), the PLP forms of H340A (lane 5), C398A (lane 6), and the H340A/C398A double mutant (lane 7); reaction mixture without enzyme (lane 8); and a mixture of the ALA and glycine standards (lane 9). (C) Far-UV CD spectra of the PLP form (line 1) and the heme form (line 2) of cALAS were measured in 50 mM sodium phosphate buffer (pH 7.4) at 20 °C. The spectra were measured at protein concentrations of 7–10 μM in a 0.2 cm light-path cell. (D) Thermal scan profiles of the PLP form (line 1) and the heme form (line 2). Thermal denaturation of each form of cALAS was irreversible.