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. 2018 Sep 21;8:14228. doi: 10.1038/s41598-018-32591-z

Figure 4.

Figure 4

Spectroscopic characterization of the heme form of cALAS. (A) UV/Vis absorption spectra of the heme form of purified cALAS (solid line (line 1)), the reduced form (dashed line (line 2)), and the reduced CO-bound form (dotted line (line 3)). The inset shows an enlarged image in the region 490–625 nm. (B) CD spectra in the visible region for the heme form of cALAS. CD spectra of the heme form of cALAS were measured in the absence (upper) and presence (lower) of 100 mM glycine. (C) The absorption spectra of the heme-form of cALAS (line 1) and the dithionite-reduced pyridine-hemochrome (line 2). For A–C, the buffer system was 50 mM HEPES-NaOH (pH 7.5) containing 150 mM KCl and 0.1 mM EDTA. The measurements were performed at 25 °C at a protein concentration of 3 μM (based on monomeric state). The concentration of heme was estimated from line 2 of panel C was 2.6 μM.