Fig. 4.

beta cell BCL11A regulates multiple pathways associated with human insulin secretion. a Workflow employed to generate RNA-seq libraries. b Schematics of lentiviral construct used to knockdown BCL11A expression. c FACS scheme used to obtain GFP expressing beta cells. d BCL11A mRNA expression in GFP expressing beta cells post FACS, control (white bar) or BCL11A-kd (black bar) (n = 4). e Pearson correlation coefficient matrix of all RNA-seq samples used in this study. f Fold transcript levels of genes critical for the maintenance of beta cell identity and function (n = 4). g GO terms enrichment in genes mis-regulated in beta cells post-BCL11A knockdown. h Fold transcript levels of genes significantly altered in beta cells post-BCL11A knockdown (n = 4). See also Supplementary Figure 4. The data presented as mean, error bars represent the standard error, and two-tailed t tests were used to generate p values. *p < 0.05, **p < 0.01