Fig. 3.

16S rRNA expression of the ¹³C-incorporating OTUs during the incubation with ¹³C-labeled 1,4-dioxane. a–d Estimated 16S rRNA molecules from the ¹³C-incorporating OTUs in the SIP experiment. Genera of the closest relatives of the OTUs are indicated in the parentheses of explanatory notes. 16S rRNA molecules from the OTUs were estimated by the total bacterial 16S rRNA copy numbers (copies ml⁻¹) and relative abundances (%) of the OTUs that were determined by RT-qPCR (n = 3) and Illumina sequencing (n = 3), respectively. The details of the Illumina sequence libraries are summarized in Table S2