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. 2018 Sep 21;9:3846. doi: 10.1038/s41467-018-06368-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — Cx43 carboxy-terminal and BTF3 form a transcriptional complex. a Diagram (top) summarizing the localization of BTF3 and Cx43 in the nucleus of st23 neural crest (bottom). Anti-BTF3 (magenta), Anti-Cx43 (green) and DAPI (blue). Scale bar = 15 μm. b Diagrams of the BiFC system. BiFC results showing that BTF3 and Cx43-20k interact in the nucleus of st23 neural crest. VENUS components coexpressed on neural crests. Scale bar = 20 μm. c Intensity of VENUS showing the efficiency of the BiFC system in the different treatments (top) and nucleus to cytosol ratio (bottom) of BiFC signal (n_TruncVC = 150, n_Cx43-20kVC = 147, n_VC = 98 cells, N = 3). d Co-immunoprecipitation of BTF3 and Cx43 in HeLa cells (N = 4). e Co-immunoprecipitation showing biochemical interaction between BTF3 and Polymesare II (N = 3). f, g ChIP experiments for n-cad promoter of st23 neural crest as indicated (f: N = 3, g: N = 3). Histograms in c, f, and g represent mean ± SE (one-way ANOVA p < 0. 001, two-tailed t- test p^** < 0.01, p^*** < 0.001). N number of independent experiments; n sample size. Spread of data in bar charts is shown as overlying dots. n.s. nonsignificant