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. 2018 Sep 21;9:3846. doi: 10.1038/s41467-018-06368-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Nuclear localization of Cx43-20k and N-cad expression is BTF3 dependent in HeLa cells. a, b siRNA efficiency. HeLa cells were transfected with siRNA control (siCTL) and siRNA against BTF3 (siBTF3). siBTF3 leads to an efficient reduction in Btf3 mRNA as analyzed by qPCR (a) (N = 4) and BTF3 protein as analyzed by western blot (b) (N = 4). c, d Cx43-20k nuclear localization. c siBTF3 leads to a strong reduction in Cx43-20k nuclear localization, compared with siCTL (N = 3). d No difference between siCTL and siBTF3 was observed in cells transfected with a control GFP vector (N = 3). Scale bar = 20 μm. e, f N-cadherin expression. siBTF3 leads to a strong reduction of the n-cadherin mRNA (N = 4) (e) and protein levels (N = 4) (f) induced by Cx43-20k-GFP, as indicated by qPCR and western blot analyses. Histograms in a, b, e, and f represent mean ± S.E. (one-way ANOVA for e p^*** < 0.001, two-tailed t- test p^** < 0.01, p^*** < 0.001, p^**** < 0.0001). N number of independent experiments; n sample size. Spread of data in bar charts is shown as overlying dots. n.s. nonsignificant