Fig. 1.
Biochemical cycle of cardiac myosin. Omecamtiv mecarbil has been shown to increase the rate of phosphate release (step 5) and bias the ATP hydrolysis step (step 3) towards the post-hydrolysis M·ADP·Pi state, which is proposed to cause myosin to enter the strong binding states (red underline) more rapidly. Other biochemical steps have been previously shown through stop-flow biochemical experiments to be nearly unchanged by the presence of OM. Inset: Example optical trapping trace of the position (median filtered with 0.4 ms window) of an actin filament during one interaction with a single-myosin molecule reproduced from Fig. 2b. The step size and attachment duration of these interactions can be measured as shown