Fig. 1.
Multiple M. oppositifolius leaf extract compounds activate KCNQ2/3. a KCNQ1–KCNQ3 chimeric structure model. b KCNQ topology (two of four subunits shown). VSD voltage-sensing domain. c Structure and electrostatic surface potential (blue, positive; green neutral; red, negative) of M. oppositifolius leaf extract components. Open red circles highlight strongly negative electrostatic surface potential. d Averaged KCNQ2/3 current traces in response to voltage protocol (upper inset) when bathed in the absence (Control) or presence of M. oppositifolius leaf extract components (n = 4–16). Dashed line indicates zero current level in this and all following current traces. e, f Mean effects of leaf extract components (as in d; n = 4–16) on: e KCNQ2/3 raw tail currents at −30 mV after prepulses as indicated; f G/Gmax. Error bars indicate SEM. Red boxes indicate KCNQ2/3 activation; black box indicates KCNQ2/3 inhibition