Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 21;8:14164. doi: 10.1038/s41598-018-32589-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Evaluation of alternative pathway in macrophages from diabetic 5LO^−/− or WT mice. Resident peritoneal macrophages from WT or 5LO^−/− mice, healthy or diabetic (T1D), were collected for the analysis of Il4r (A) and Stat6 (B) expression by qPCR. Total and phosphorylated STAT6 was also analyzed by western blot (C,D). Cells were stimulated with recombinant IL-4 (4000, 400 or 40 ng), and the expression of total STAT6 and phosphorylated (p-STAT6) and β-Actin were analyzed by western blot (C–E). Full-length blots/membranes are presented in Supplementary Figures 3 (loading control), 4 (total STAT6), 5 (p-STAT6) and 6 (β-Actin). Data are presented as the mean ± SEM of 3 to 5 animals per group. *p ≤ 0.05 when the diabetic groups were compared to healthy WT, ^#p ≤ 0.05 when diabetic 5LO^−/− group was compared to diabetic WT. ^&p ≤ 0.05 when IL-4 groups were compared to control (CT) groups. H = healthy.