Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 21;8:14161. doi: 10.1038/s41598-018-32256-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Macropinocytosis is further increased by autophagy inhibition in Tsc2-deficient cells. (A) Immunoblots confirmed Atg5 knockout in Tsc2^+/+ and Tsc2^−/− MEFs. The blot was cropped to highlight the relevant bands. The full-length blot is presented in Supplementary Fig. 3. (B) Macropinocytosis was increased in Tsc2^−/− sgAtg5 MEFs compared to Tsc2^−/− sgCtl MEFs. Median fluorescent intensity of FITC-Dextran was quantified by flow cytometry. Data represented as mean +/− standard deviation of three biological replicates. Statistical significance was assessed using two-way and one-way ANOVAs with Bonferroni correction with ****p < 0.0001.