Table 4.

Summary of the proposed guidelines.

	Guidelines
1	To use more than one misfolded SOD1/conformational-specific antibody before concluding any results
2	To test and use optimal antibodies working concentrations
3	To optimize antigen retrieval time for each antibody
4	To use citrate-based instead of TRIS/EDTA-based buffers
5	To test different central nervous system regions (cervical, lumbar, thoracic spinal cord sections and other brain regions)
6	To perform, in parallel, Hematoxylin/Eosin coloration on adjacent sections