Table 1.
Inhibition of PCA by anti-IgE antibodies.
| anti-DNPIgE vs | Antibodies, (μg/ml) | ||||||
|---|---|---|---|---|---|---|---|
| 20 | 10 | 5 | 2.5 | 1.25 | 0.63 | 0 | |
| Fab-6HD5 | − | − | − | − | ± | + | +++ |
| 6HD5 | − | − | − | − | + | ++ | +++ |
| Fab-HMK12 | − | − | + | ++ | ++ | +++ | +++ |
| HMK12 | − | − | + | ++ | ++ | +++ | +++ |
| Rat IgG | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| anti-TNPIgE vs | Antibodies, (μg/ml) | ||||||
| 20 | 10 | 5 | 2.5 | 1.25 | 0.63 | 0 | |
| Fab-6HD5 | − | − | − | − | ± | + | +++ |
| Fab-HMK12 | − | − | + | ++ | ++ | +++ | +++ |
| Anti-κ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Rat IgG | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Serial dilutions of anti-DNP IgE (SPE-7) or anti-TNP IgE (142a) were injected intradermally into rats. Twenty-four hours later, each antibody (Fab-6HD5, Fab-HMK-12, Fab-anti-κ, or rat IgG) was injected into the same site. Two hours after the second series of injections, the rats were injected intravenously with 0.5% Evans blue with DNP-BSA or TNP-BSA. The reactions (the diameters of the blue spots) were measured 30 min later. +++, blue spot of >15 mm in diameter; ++, ≥10 mm in diameter; +, <10 mm in diameter; ±, <5 mm in diameter; −, no visible blue spot.