Table 2.

Time course studies of PCA inhibition by 6HD5.

Evans Blue	Fab-6HD5, (μg/ml)
extravasation	20	10	5	2.5	1.25	0.63	0.32	0.16	0
Day 1	−	−	−	−	±	±	+	++	+++
Day 10	−	−	−	−	−	±	±	+	+++

PCA day 1: SPE-7 (anti-DNP IgE) was injected intradermally into rats on day 0, and a serial dilution of Fab-6HD5 was injected into the same sites on day 1. Two hours later, an Evans blue extravasation assay was performed.

PCA day 10: an Evans blue extravasation assay was performed on day 10. +++, blue spot of >15 mm in diameter; ++, ≥10 mm in diameter; +, <10 mm in diameter; ±, <5 mm in diameter; −, no visible blue spot.