Fig. 6. Antitumor effect in a B16 lung metastases model.
Some 2 × 105 B16 cells were injected i.v. into C57BL/6 mice in each group (n > 5/group) on day 0. Then 20 μg of FSD13 and IL-2 was injected i.p. to treat mice bearing tumor cells. PBS served as control. After mice were killed, the metastasic nodules on lung were counted and the biggest nodule was measured. a Procedure represents the experiments. b, c, d FSD13 exhibited stronger antitumor effects than IL-2. All experiments were repeated three times. Experiments were conducted in more than six biological replicates (n = 6) for each group. Data were analyzed by Student’s t-test and represented as mean ± SEM, and were collected from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001