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. 2018 Sep 24;9(10):971. doi: 10.1038/s41419-018-1039-2

Fig. 6. GRP75 knockdown impairs CHIP expression and its binding to proto-Dbl.

Fig. 6

a GRP75 KD (G3) and NC Cos-7 stable cell lines were co-transfected with GST-Dbl constructs and myc-CHIP plasmids. Exogenous GST-proto-Dbl were pull-down from cell lysate by mouse anti-GST Ab, and co-pull-down CHIP, Hsc70, and HSP90 were evaluated by their corresponding Ab, respectively. b GRP75 KD (G3) Cos-7 stable cells were co-transfected with GST-Dbl constructs and myc-CHIP plasmids. 48 h post-transfection, exogenous GST-Dbl were immunoprecipitated from cell lysates by mouse anti-GST Ab, and co-immunoprecipitated CHIP was evaluated by rabbit anti-Myc Ab. The lysate of the NC Cos-7 stable cell line was included as the control (NC). c The expression level of endogenous CHIP, proto-, and onco-Dbl among SKOV-3, GRP75 KD (G2), and NC SKOV-3 stable cell lines were compared by Western blot. Representative blot results from three independent experiments are shown