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. 2018 Sep 24;8:14269. doi: 10.1038/s41598-018-32549-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs (x20) of representative hematoxylin and eosinofil stained slides of histopathology of prostate tissue cryosections with reactive stroma grade (RSG) 0–3. (a) Normal prostatic tissue with reactive stromal grade (RSG) 0 (<5% reactive stroma). Stroma is mostly consisting of smooth muscle cells making up bundles. (b) RSG 1 (6–15% reactive stroma) and Grade Group 4. The majority of stroma still has a strong eosinophilic stain, with a few cells with paler staining appearing, in addition to the presence of more fibroblasts. (c) RSG 2 (16–50% reactive stroma) and Grade Group 3. The reactive stroma is more prominent by a weaker eosinophilic stain. (d) Sample with RSG 3 (>50% reactive stroma) and Grade Group 3. Here, nearly all normal stroma is replaced by reactive stroma with pale eosinophil staining.