Fig. 5. ARHGAP5 downregulation suppresses migration, invasion, and metastasis of GC in vitro and in vivo.

a Effective knockdown of ARHGAP5 by siRNA was examined by qPCR and western blot. b Cell migration and invasion were evaluated by Transwell assays without or with Matrigel in AGS and BGC-823 cells transfected with the indicated siRNAs. Scale bars, 100 µm. c Statistical analyses of migrating or invasive cells per visual field. d The weight of lungs was evaluated. e Representative pictures of lungs from each group. f HE staining of lung tissues with metastatic nodules. Scale bars, 200 µm in the upper panel and 100 µm in the lower panel. All data indicate the mean ± SD. ***p < 0.001