Fig. 6. ARHGAP5 is involved in SIRT1-induced suppression of GC cell migration and invasion.

a Effective knockdown of ARHGAP5 in stably lentivirus-infected AGS and BGC-823 cells with SIRT1 depletion and their controls. Ni for control siRNA and Ai for siRNA targeting ARHGAP5. b Cell migration and invasion were evaluated by Transwell assays without or with Matrigel in stably lentivirus-infected AGS and BGC-823 cells (LV-Ci and LV-Si) transfected with the indicated siRNAs. Scale bars, 100 µm. The statistical analyses of migrating or invasive cells per visual field were presented as histogram in c. d The weight of lungs was evaluated. e Representative pictures of lungs from each group. f HE staining of lung tissues with metastatic nodules. Scale bars, 200 µm in the upper panel and 100 µm in the lower panel. All data indicate the mean ± SD. ***p < 0.001