Fig. 7.

In the absence of both RAD52 and BRCA2, ssDNA/RAD51 interactions are severely abrogated. a Quantification of RAD51 association with repair foci following CPT treatment and release (0, 2 h) in BRCA1/RAD52-depleted cells. b Quantification of BRCA2 association with repair foci following CPT treatment and release (0, 2 h) in RAD52- and BRCA1/RAD52-depleted cells (RAD52-depleted cells shown to contrast increased BRCA2 levels required for ssDNA/RAD51 interactions). c Representations of the HR repair pathway deficiency in RAD52/BRCA1-depleted cells and the concluded HR repair pathway in vivo in WT cells. For complete N values, see Supplementary Table 1. Error bars represent mean ± s.e.m. Student’s t test for significance between WT + CPT and siRAD52/siBRCA1+CPT and siRAD52+CPT and siRAD52/siBRCA1+CPT as indicated. ****p < 0.0001