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. 2018 Sep 24;8:14299. doi: 10.1038/s41598-018-32653-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of CD298+ tumor cells in the bone using flow cytometry. (a) Representative flow cytometry plot of CD298 staining in non-tumor-inoculated (naïve) mouse bone marrow used as a negative control for the MCF7 model (representative of n = 3 mice). Dead cells (PI+) have been excluded. (b) Representative flow cytometry plots of bone marrow from MCF7-inoculated −E2 (n = 10 mice) and +E2 (n = 8 mice) mice. Dead cells (PI+) have been excluded. (c,d) Quantitation of total number and percent of CD298+ tumor cells from (b). (e) Representative flow cytometry plot of CD298 staining in non-tumor-inoculated (naïve) mouse bone marrow used as a negative control for the SUM159 model (representative of n = 3 mice). Dead cells (PI+) have been excluded. (f) Representative flow cytometry plot of bone marrow from SUM159-inoculated mice (n = 8 mice). Dead cells (PI+) have been excluded. (g,h) Quantitation of total number and percent of CD298+ tumor cells from (f). (c) Mann-Whitney U-Test, *P < 0.05 and **P < 0.01.