Fig. 3. Knockdown of POFUT1 significantly inhibited CRC proliferation in vitro.

a Compared negative control group (NC). Knockdown of shPOFUT1 (shPO) significantly reduced CRC cell proliferation ability by CCK8 assay in SW620 and HCT116 cell lines. **p < 0.03. b Compared negative control group (NC). Knockdown of shPOFUT1 (shPO) decreased CRC cell colony numbers in SW620 and HCT116 cell lines. Quantification (right panel), *p < 0.05. c POFUT1 arrested the progression of the cell cycle in SW620 and HCT116 cell lines. Quantification (right panel), these data were detected by a flow cytometry. *p < 0.05, **p < 0.03. d Western blot assay revealed the level of p53 increased and the level of protein CCND1 and c-Myc decreased in the POFUT1 group compared with the NC group. *p < 0.05