Fig. 6.

The recruitment capacity of iNKT cells is regulated by Med23. a The absolute numbers of neutrophils, monocytes, macrophages, T cells, B cells and NK cells in the livers of eight-week-old WT mice injected with 2 μg of α-GalCer at various time points (neutrophil, 0 h and 2 h, n = 4; 4 h and 6 h, n = 5) (monocyte, 0 h, 4 h and 6 h, n = 4; 2 h, n = 5) (macrophage, 0 h and 6 h, n = 4; 2 h and 4 h, n = 5) (T cell, 0 h, n = 4; 2 h and 6 h, n = 5; 4 h, n = 6) (B cell, 0 h, 4 h and 6 h, n = 4; 2 h, n = 5) (NK cell, 0 h, n = 4; 2 h, 4 h and 6 h, n = 5). b The transcriptional levels of Xcl1, Ccl5, and Ccl4 in sorted WT stage 2 and stage 3 cells and Med23^−/− stage 2 cells from the liver 1 h after injection of 2 μg of α-GalCer. Gene expression was normalized to Gapdh expression (n = 5). c, d Production of CCL5 in WT stage 2 and stage 3 cells and Med23^−/− stage 2 cells of the spleen (c) and liver (d) 1 h after the injection of 2 μg of α-GalCer or mock. e The percentage of CCL5⁺ cells among WT stage 2 and stage 3 cells and Med23^−/− stage 2 cells in the spleens and livers 1 h after the injection of 2 μg of α-GalCer (n = 5). The data are presented as the mean ± s.d. For all panels: *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t-test; N.S. no significance. All data are representative of or combined from at least three independent experiments