Fig. 4. b-AP15 or PtPT induce cell cycle arrest in BCa cells.

a BCa cells were exposed to b-AP15 for 24 h. Cells were fixed and stained with PI followed by flow cytometry. Representative images and (b) distribution of cell cycle stages is shown. c BCa cells were exposed to PtPT for 24 h. Cells were fixed and stained with PI followed by flow cytometry. Representative images and (d) distribution of cell death are shown. e BCa cells were treated with indicated concentrations of b-AP15, or b-AP15 (1 μM) for indicated time. Total proteins were extracted. The expression of CDK4, cyclinD1 and P21 were tested by western blot. GAPDH was shown as a loading control. f BCa cells were treated with indicated concentrations of PtPT, or PtPT (5 μM) for the indicated time. Total proteins were extracted. The expression of CDK4, cyclinD1 and P21 were tested by western blot. GAPDH was probed as a loading control