. 2018 Sep 24;8:14273. doi: 10.1038/s41598-018-32687-6

Table 2.

Results of in vitro development of separated oocytes by various methods followed by IVF.

Method		Number of oocytes	Number of blastocysts				Developmental rate to blastocyst (% ± SEM)
Method		Number of oocytes	Early blastocyst	Blastocyst expansion	Hatched blastocyst	Total	Developmental rate to blastocyst (% ± SEM)
Microfluidic	Upper	163	11	11	1	23	14.1 ± 1.3^a
device	Lower	197	18	43	10	71	36.0 ± 1.2^b
BCB	BCB−	143	12	9	1	22	15.4 ± 0.7^ac
staining	BCB+	213	23	42	11	76	35.7 ± 1.2^b
Conventional	A	412	31	47	7	85	20.6 ± 1.1^c
morphological	B	375	38	86	6	130	34.7 ± 0.7^b
evaluation	C	300	30	44	11	85	28.3 ± 1.5^d

Oocytes collected from both upper and lower outlets were prepared for IVF and cultured, and their developmental rates to blastocyst stage were investigated on day 8. As a comparison method, BCB staining was performed to compare the separation capability between separation methods. The data were obtained from three replicates. Conventional morphological evaluation was performed by three different technicians (A, B, C) as a comparison method. COCs with a homogeneous spherical ooplasm and multilayered compact cumulus cells were selected by each technician and prepared for IVM and IVF. All technicians performed all procedures using the same protocols and materials. The data were obtained from six replicates. Values with different letters differ significantly, P < 0.01.