Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 24;8:14294. doi: 10.1038/s41598-018-32428-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Skp2 is overexpressed in osteosarcoma cell lines and high Skp2 levels are correlated with a worse prognosis. (A) Quantitative RT-PCR. Skp2 mRNA expression in 5 standard and 8 patient-derived osteosarcoma cell lines was significantly increased compared to normal human osteoblasts (NHOst). (B,C) Skp2 protein levels were elevated in standard (B) and patient-derived (C) osteosarcoma cell lines compared to NHOsts. (D) Kaplan-Meier analysis. Raw Skp2 expression data was retrieved from NCBI GEO and correlated with survival data from the R2 platform. The median Skp2 mRNA expression was used as a cutoff to distinguish low vs. high expression. High Skp2 expression correlated significantly with a worse metastasis-free survival. (E) Tissue microarrays. Overall survival was compared in osteosarcoma patients whose tumors expressed low (- and +) vs. high (++ and +++) Skp2 (negative =<1% stained cells; (+) = 1–10%; (++) = 10–50%; (+++) =>50%). By log-rank test, the high Skp2 expression group sustained a worse overall survival than the low expression group. (F) Representative pictures of IHC scoring for Skp2. Statistical significance is indicated by: *p < 0.05, **p < 0.01, ***p < 0.001. Column: mean; Error bars: SD.