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. 2018 Sep 24;9(10):987. doi: 10.1038/s41419-018-1001-3

Fig. 4. G9a modulated the methylation of SOSTDC1 promoter.

Fig. 4

a G9a expression in TC from TCGA database. b Interaction between SOSTDC1 promoter and G9a in K1 cells and Nthy-ori 3-1 cells. c The SOSTDC1 luciferase reporter activity in K1 and 8505C cells transfected with control or G9a siRNAs. d K1, 8505C, and normal Nthy-ori 3-1 cells transfected with control or G9a siRNAs for 72 h, and analysis of promoter methylation of SOSTDC1. e Interaction between SOSTDC1 promoter and DNMT1 in K1 cells with or without G9a siRNA. f Proteins expression in K1 and 8505C cells transfected with control or G9a siRNAs for 72 h. g mRNA expression of SOSTDC1 in K1 and 8505C cells transfected with control or G9a siRNAs for 72 h. h Secretion of hepcidin in K1 and 8505C cells transfected with control or G9a siRNAs for 72 h. i The proliferation of K1 and 8505C cells transfected with control or G9a siRNAs for 72 h was analyzed by MTT assay. j The proliferation of K1 and 8505C cells transfected with control or G9a siRNAs for 72 h was analyzed by BrdU assay. Each experiment was repeated for three times. *P < 0.05; **P < 0.01