Fig. 2. Changes of SQSTM-1/p62 levels following retinal ischemia reperfusion.

A Western blotting analysis reporting the reperfusion time-dependent modulation of SQSTM-1/p62. SQSTM-1/p62 expression decreased in the ischemic retina as compared with contralateral after 6 h of reperfusion, whereas accumulated at 24 h. Histograms show the densitometric analysis of the bands normalized to loading control (actin) and reported as mean ± s.e.m. (3–6 independent experiments for each group). Dashed line indicates the baseline expression of the protein of interest in control non-ischemic retinas set to 1. ^#P < 0.05 vs C (Student’s t test); *P < 0.05, **P < 0.01 (ANOVA followed by Tukey–Kramer for multiple comparisons test). C, control non-ischemic retina; I, ischemic retina; MW, molecular weight; Rep time, reperfusion time. B Representative retinal tissue sections showing SQSTM-1/p62 immunoreactivity in control and ischemic retinas after 24 h of reperfusion. C Colabeling of ischemic retina at 24 h reperfusion with anti-p62 (green) and anti-TUJ1 (red), a RGC-specific marker, demonstrating p62 upregulation in RGC soma (GCL) and dendrites (IPL). Nuclei were counterstained with DAPI (blue). Images are representative of three animals per experimental conditions ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars B 50 μm; C 47.62 μm