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. 2018 Sep 24;9(10):983. doi: 10.1038/s41419-018-1023-x

Fig. 2. Requirement for caspase-4/5/11 in mediating contrast-induced IL-1β cleavage and pyroptosis.

Fig. 2

a Immunoblot analysis of the pro-IL-1β and mature IL-1β in TECs isolated from WT and Casp11–/– mice treated with extracellular iohexol (20 mg/ml) for 72 h and the two duplicate samples represented separate study of TECs isolated from WT and Casp11–/– mice treated with extracellular iohexol. b Quantification of the pro-IL-1β and mature IL-1β expression shows significant upregulation in TECs from WT mice, whereas the response in mature IL-1β was blocked in TECs from Casp11–/– mice. LDH release by human TECs (c), mRNA expression of IL-1β determined by real-time PCR (d), and mature IL-1β in human TEC culture supernatants (e) after a 72 h period of iohexol (20 mg/ml) or isosmotic mannitol incubation were blocked by knockdown (KD) of the human inflammatory Casp4/5. Experiments were performed in triplicate. Ctrl, control; Casp 4/5, caspases 4/5; KD, knockdown. **P < 0.01, ***P < 0.001 vs. control or as indicated. Data are means ± SEM. Statistics obtained from ANOVA