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. 2018 Sep 24;8:14307. doi: 10.1038/s41598-018-32460-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overall structure of FlgL. (a) Schematic diagram that shows the arrangement of domains and secondary structures in bcFlgL and xcFlgL. (b,c) Structures of bcFlgL (b) and xcFlgL^C2 (c). The FlgL structures are shown in rainbow-colored ribbons (N-terminus, blue; C-terminus, red). The secondary structures that bcFlgL and xcFlgL share are highlighted with magenta labels. (d) Overlay of the bcFlgL (green ribbons) and xcFlgL (light blue ribbons) structures. The common secondary structures of bcFlgL and xcFlgL are labeled in magenta. The inset shows that the β1 and β2 strands of bcFlgL are positioned differently from those of xcFlgL. (e) Conserved four-segment (S1–S4) arrangement in the D1 domains of bcFlgL and xcFlgL. The S1, S2, S3, and S4 segments are represented by the secondary structures of α1, α2, β3/β4/α3, and α4, respectively. (f) Topology diagram of the D1 domain in the FlgL structures.