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. 2018 Sep 24;8:14272. doi: 10.1038/s41598-018-32690-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MyD88-dependent LTβR stimulation-induced expression of TLO-associated genes and signal transduction. (a) qRT-PCR analysis of Lta, Ltb, and Ltbr expression in whole SMGs from 12-week-old female Myd88^+/+ and Myd88^−/− NOD mice (n = 6 per genotype). Expression levels calculated relative to Hprt expression. Results are expressed as mean ± SD (bar graph). *p < 0.05. (b) Myd88^+/+ and Myd88^−/− MEFs were stimulated with 2.5 μg/mL LTβR agonistic antibody for indicated periods, followed by total RNA extraction. Relative mRNA expression of Glycam1, Cxcl13, Ccl19, Tnf, Map3k14, and Ltbr was determined by qRT-PCR. Expression levels were calculated relative to Hprt expression. Representative results of three independent experiments are shown. (c) Myd88^+/+ and Myd88^−/− MEFs were stimulated with 2.5 μg/mL LTβR agonistic antibody for indicated periods, followed by lysis, SDS-PAGE and immunoblotting to assess levels of NIK, NF-κB2 (p100/p52), TRAF3, and Tubulin. Representative results of at least three independent experiments are shown. All the blots were obtained under the same experimental conditions, and the cropped images of the blots are shown. The uncropped images are in Supplementary Fig. 8.