Figure 2.

Induction of nuclear condensation in Caco2 cells by melatonin or 2-hydroxymelatonin. (a) Hoechst 33258 staining of Caco2 cells treated with melatonin or 2-hydroxymelatonin dissolved in ethanol. Arrows indicate cells with condensed or fragmented nuclei. Representative images from three independent experiments are shown. Enlarged images are shown in the top right inset of the image; (b) Quantification of condensed or fragmented nuclei in cells treated with melatonin or 2-hydroxymelatonin. Data represent mean ± standard error of the mean, n = 3. EtOH, ethanol; M, melatonin; 2-OH-M, 2-hydroxymelatonin.