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. 2017 Mar 11;22(3):451. doi: 10.3390/molecules22030451

Table 6.

Linear relation between peak area and concentration (n = 3).

Compound Number Rt (min) Regression Equation r2 Linear Range (μg/mL) LOD (μg/mL) LOQ (μg/mL)
3 15.479 y = (2.0 × 106)x + 13710 1.0000 1 - 250 0.96 2.92
4 17.416 y = (2.0 × 106)x − 16531 1.0000 1 - 500 0.80 2.42
6 38.645 y = (2.0 × 106)x + 26229 0.9997 1 - 500 2.78 8.44
8 45.640 y = (7.0 × 106)x + 26716 0.9998 1 - 500 0.77 2.33

In the regression equation y = ax + b, x refers to the concentration of the compound (μg/mL), y the peak area; r2: the correlation of the equation; Rt: retention time; LOD: limit of detection; LOQ: limit of quantification.