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. 2017 Mar 22;22(3):504. doi: 10.3390/molecules22030504

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of Shenqi on protein expression of T-bet, GATA3, p-STAT6, and SOCS1 in OVA-stimulated lymphocytes. Primary spleen lymphocytes isolated from Wistar rats were preincubated in the presence or absence of Shenqi (50, 100, and 200 μg/mL) or Loratadine (5 μM) for 1 h and then cultured with OVA for another 72 h. The total cell proteins were extracted to detect the expression of Th1 regulating factor T-bet (A); Th2 regulating factor GATA3 (B) and phosphor-STAT6 (C), as well as the negative regulator SOCS1 (D) via Western blot. The data are presented as the means ± SEM of three independent experiments, ^# p < 0.05, ^## p < 0.01 vs. cells alone, * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. OVA-treated cells.

Effects of Shenqi on protein expression of T-bet, GATA3, p-STAT6, and SOCS1 in OVA-stimulated lymphocytes. Primary spleen lymphocytes isolated from Wistar rats were preincubated in the presence or absence of Shenqi (50, 100, and 200 μg/mL) or Loratadine (5 μM) for 1 h and then cultured with OVA for another 72 h. The total cell proteins were extracted to detect the expression of Th1 regulating factor T-bet (A); Th2 regulating factor GATA3 (B) and phosphor-STAT6 (C), as well as the negative regulator SOCS1 (D) via Western blot. The data are presented as the means ± SEM of three independent experiments, ^# p < 0.05, ^## p < 0.01 vs. cells alone, * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. OVA-treated cells.