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. 2017 Mar 10;22(3):437. doi: 10.3390/molecules22030437

Figure 2.

Figure 2

Bufalin induced cell cycle arrest in U-2 OS cells. Cells were treated with 200 nM of bufalin for 0, 12, 24, and 48 h and then were stained with PI (Propidium iodide) for cycle distribution by flow cytometric assay, as described in Materials and Methods. (A) Representative profiles and (B) percentage of cell cycle distribution. Different letters mean significant difference between bufalin-treated groups and the control as analyzed by one-way analysis of variance (ANOVA), followed by Duncan’s multiple range test (DMRT).