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. 2017 Mar 10;22(3):437. doi: 10.3390/molecules22030437

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bufalin affects the activities of caspase-3, -9, and -8 in U-2 OS cells. U-2 OS cells (1 × 10⁵ cells/well) were incubated with 200 nM of bufalin for 0, 12, 24, and 48 h; then the cells were harvested and re-suspended in 25 μL of 10 μM substrate solution containing (A) PhiPhiLux-G1D1 for caspase-3, (B) CaspaLux9-M1D2 for caspase-9, and (C) CaspaLux8-L₁D₂ for caspase-8 activity measurements, as described in Materials and Methods. The results are shown as a mean ± SD (n = 3); * p < 0.05, significant difference between bufalin-treated groups and the control as analyzed by Student’s t test.

Bufalin affects the activities of caspase-3, -9, and -8 in U-2 OS cells. U-2 OS cells (1 × 10⁵ cells/well) were incubated with 200 nM of bufalin for 0, 12, 24, and 48 h; then the cells were harvested and re-suspended in 25 μL of 10 μM substrate solution containing (A) PhiPhiLux-G1D1 for caspase-3, (B) CaspaLux9-M1D2 for caspase-9, and (C) CaspaLux8-L₁D₂ for caspase-8 activity measurements, as described in Materials and Methods. The results are shown as a mean ± SD (n = 3); * p < 0.05, significant difference between bufalin-treated groups and the control as analyzed by Student’s t test.