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. 2017 Mar 10;22(3):437. doi: 10.3390/molecules22030437

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bufalin affects apoptosis associated protein expression in U-2 OS cells. Cells were treated with 200 nM of bufalin for 0, 6, 12, 24, and 48 h, and then the total proteins were quantitated and the apoptosis associated proteins were measured by Western blotting, as described in Materials and Methods. (A) Bcl-2, Bcl-xl, Bax, cytochrome c, AIF and Endo G; (B): caspase-8, -9 and -3, Fas, Fas-L, and cleaved PARP (poly (ADP-ribose) polymerase); (C): Calpain 1, ATF-6α, Caspase-4, GRP-78 and GADD153.

Bufalin affects apoptosis associated protein expression in U-2 OS cells. Cells were treated with 200 nM of bufalin for 0, 6, 12, 24, and 48 h, and then the total proteins were quantitated and the apoptosis associated proteins were measured by Western blotting, as described in Materials and Methods. (A) Bcl-2, Bcl-xl, Bax, cytochrome c, AIF and Endo G; (B): caspase-8, -9 and -3, Fas, Fas-L, and cleaved PARP (poly (ADP-ribose) polymerase); (C): Calpain 1, ATF-6α, Caspase-4, GRP-78 and GADD153.