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Bufalin affects the translocation of apoptotic-associated proteins in U-2 OS cells. Cells were treated with 200 nM of bufalin for 48 h, and cells were stained by (A) anti-Endo G, (B) -cytochrome c, and (C) -AIF and then were stained with a secondary antibody and were examined and photographed by a Leica TCS SP2 confocal laser microscopic system, as described in Materials and Methods.
