Figure 3.
Level of H2S formed in vitro non-enzymatically from LA protected or non-protected from light detected by diffusion assay. In addition, 10 mM solution of LA in 50 mM phosphate buffer was used fresh as well as after three or seven days left in the presence or absence of environmental light. On the day of the experiment, the LA samples were pipetted into diffusion chambers and the level of H2S was detected after 90 min of diffusion. (A) the level of H2S formed at pH 8.0 from fresh LA, three-days LA and seven-days LA as well as from 100 µM and 1 mM Na2S as the controls; and (B) the level of H2S formed at pH 5.0 from three-days LA and seven-days LA. The results are presented as the means ± SD from samples run in triplicate.