Table 1.
MRM parameters, chromatographic attributes, and quantitative response of stilbene compounds in standard samples.
| Compound | Formula | Mass (Da) | Precursor Ion (m/z) | Product Ion (m/z) | Collision Energy (eV) | % Intensity | Retention Time (Min–Max) trans/cis | LOD (mg/L) | LOQ (mg/L) | Linearity b |
|---|---|---|---|---|---|---|---|---|---|---|
| Piceid | C20H22O8 | 390.13 | 391.1 | 114.8 | 20 | 86.5 | 0.93–0.99/1.52–1.63 | 0.04 | 0.07 | 640 |
| 308.8 | 8 | 67.5 | ||||||||
| 349.9 | 0 | 100 | ||||||||
| 229.1 a | 8 | 24.4 | ||||||||
| Resveratrol | C14H12O3 | 228.08 | 229.09 | 107.1 a | 24 | 100 | 1.85–1.99/2.55–2.65 | 0.12 | 0.22 | 800 |
| 91 a | 24 | 45.7 | ||||||||
| 135 a | 8 | 80.9 | ||||||||
| 165 a | 28 | 35.6 | ||||||||
| Piceatannol | C14H12O4 | 244.07 | 245.08 | 107.1 | 20 | 95.5 | 1.25–1.35/1.90–2.00 | 0.12 | 0.15 | 1600 |
| 135.1 | 12 | 100 | ||||||||
| 152 | 36 | 11.6 | ||||||||
| 181.1 | 24 | 38.7 | ||||||||
| ε-Viniferin | C28H22O6 | 454.14 | 455.15 | 107.1 | 32 | 100 | 3.00–3.17/2.80–2.90 | 0.07 | 0.09 | 1777 |
| 215.1 | 20 | 75.0 | ||||||||
| 349.1 | 16 | 38.5 | ||||||||
| 199.1 | 24 | 32.0 | ||||||||
| Pteroestilbene | C16H16O3 | 256,11 | 257.12 | 181 | 40 | 90.1 | 5.18–5.28/5.26–5.35 | 0.06 | 0.08 | 1000 |
| 133.1 | 12 | 100 | ||||||||
| 91 | 28 | 58.5 | ||||||||
| 165.1 | 40 | 72.2 |
a Transitions used for piceid analysis. b Ratio between the highest and lowest concentrations in linear range.