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. 2017 Mar 13;22(3):457. doi: 10.3390/molecules22030457

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Response surface for the effects of independent variables on encapsulation efficiency and size of C3G nanoliposomes. The effects of phosphatidylcholine-to-cholesterol ratio and temperature on size are shown in (A) (C3G concentration = 0.15 mg/mL); The effects of C3G concentration and rotary evaporation temperature were shown in (B) (phosphatidylcholine-to-cholesterol ratio = 3); The effects of phosphatidylcholine-to-cholesterol ratio and temperature on EE (%) are shown in (C) (phosphatidylcholine-to-cholesterol ratio = 3); The effects of phosphatidylcholine-to-cholesterol ratio and C3G concentration were shown in (D) (rotary evaporation temperature = 40 °C).