Figure 5. Axonal Growth Is Linear and Reduced by 20% in Absence of TUBB3.

(A) Left: high magnification of neurite outgrowth in Tubb3^−/− versus wild-type spot cultures at DIV4 and DIV8. Scale bar, 50 mm. Right: quantification of Sholl analysis. The black and green lines represent WT and Tubb3^−/− growth, respectively. n = 8. Scale bar, 50 μm.

(B) Quantifications of growth of naive versus pre-conditioned wild-type and Tubb3^−/− DRG neurons on poly-D-lysine neutral substrate and on CSPG inhibitory substrate. *p < 0.05. One-way ANOVA, post hoc Sidak’s. n = 12 wells per condition.

(C) Quantile-quantile curve of Tubb3^−/− neurite growth normalized to wild-type samples. Green dots represent neurite growth for each Tubb3^−/− Tubb3^−/− DRG neuron calculated by interpolation. Dashed black line represents WT growth. Dashed-red line is the linear regression of Tubb3^/ neurite outgrowth. n = 486 neurons.

Error bars indicate SEM. See also Figure S4.