Figure 7.

GRP78 overexpression decreases TON-induced tau phosphorylation and oligomerization. At 4 to 5 weeks after intravitreal injection of AAV2-Null or GRP78, mice were subjected to TON. Eyes were collected for frozen sections 3 days after TON. Representative images of phosphorylated tau (A) and tau oligomer (B) (green) are shown. Arrowheads indicate nonspecific vessel staining. Bar graphs represent quantification of immunoreactivity of phosphorylated tau and tau oligomer. n = 3–4. *P < 0.05 AAV2-Null-Con versus AAV2-Null-TON, #P < 0.05 AAV2-Null-TON versus AAV2-GRP78-TON. Scale bar: 50 μm.