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A) Albumin to creatinine ratio (ACR) at sacrifice (22 weeks). B) ACR follow-up for 22 weeks. C) Periodic acid–Schiff (PAS) staining: a) non-DB, b) DB, c) DB EN, d) DB PYR+EN, e) DB PPS+EN, f) DB PPS+PYR+EN. D) Morphometric evaluation of glomerular PAS positive areas. E) Kidney TGFβ1 mRNA levels. *p<0.05; **p<0.01; ***p<0.005; #p<0.001. DB, diabetics; EN, enalapril; PPS, pentosan polysulfate; PYR, pyridoxamine; PAS, periodic acid–Schiff; TGFβ1, transforming growth factor β1.
