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. 2018 Sep 13;16(9):e2005895. doi: 10.1371/journal.pbio.2005895

Fig 6. Localization of candidate apicoplast proteins identified by BioID.

Fig 6

(A) Transit peptide processing assay for C-terminally GFP-tagged candidates. Ring-stage parasites were either untreated or treated with 10 μM actinonin/200 μM IPP for 3 days and protein processing was assessed by western blot. (B) Fixed-cell imaging of GFP-tagged candidates in parasites stained with an antibody raised against the apicoplast marker ACP. ROM7–GFP-expressing parasites were also stained with anti-GFP antibody due to low signal from intrinsic GFP fluorescence in fixed cells. Arrowheads indicate regions where PF3D7_0721100-GFP puncta appear adjacent to as opposed to colocalizing with ACP. Scale bars, 5 μm. ACP, acyl carrier protein; BioID, proximity-dependent biotin identification; GFP, green fluorescent protein; IPP, isopentenyl pyrophosphate; ROM, rhomboid protease homolog.