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. 2018 Sep 25;13(9):e0204567. doi: 10.1371/journal.pone.0204567

Fig 2. C. rodentium density, colonic epithelial health status and VIP staining intensity in the murine distal colon during infection.

Fig 2

A: Fecal C. rodentium density and total colitis score: C. rodentium CFU in fecal pellets from individual mice. The colitis score is presented as the sum of the scores from tissue damage, crypt architecture, crypt length, crypt abscesses, goblet cell depletion, inflammatory cell infiltration and neutrophils in lamina propria. (B) Semi-quantification (visually scored 0–5) of the VIP staining intensity in the murine distal colon of wild type (WT) and IFN-γ-/- mice. (C) Colonic epithelial health status measuring Rp (Ω/cm2) and PD (mV/cm2) of mice following day 4, 10, 14 and 19 post-infection in wild type mice infected with C. rodentium. (D-F) Representative images of distal colonic specimens immunohistochemically stained for VIP (brown). Scale bar 200 μm, magnification x200. (G-L) Colonic tissue sections co-stained with a general eubacterial probe (Eub338, red) and an antibody against E. coli lipopolysaccharide antigen O152 (green), which also stains C. rodentium. (G-I) Representative images of feces (scale bar 50 μm, magnification x400) and (J-L) epithelial surface (scale bar 50 μm, magnification x200). Statistics: data are presented as mean ± S.E.M. and analyzed using ANOVA with Student Newman-Keuls Multiple Comparison post hoc test: * P<0.05, ** P<0.01*** P <0.001 vs. control. (n = 5 mice/group). The experiments were performed twice.