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Variables in Box-Behnken design for the preparation of daidzein.
| Factors | Actual and Coded Levels Used for the Conditions | ||
|---|---|---|---|
| Low (−1) | Medium (0) | High (+1) | |
| A = Reaction time (min) | 30 | 90 | 150 |
| B = Temperature (°C) | 45 | 55 | 65 |
| C = pH value | 3.0 | 5.0 | 7.0 |
| D = Enzyme loading (U) | 0.5 | 1.5 | 2.5 |
| Dependent variable (response) | Constrains | ||
| R = Conversion yield (%) | Maximize | ||
