Table 1.
Determination of HF anticlastogenic effect towards benzo(a)pyrene using the chromosome aberration test on HepG2 cells.
| HF/B(a)P Concentration (µM) | Number of Aberrant Metaphases | Number of Chromosome Aberrations | Total Number of CA | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromatid | Isochromatid | Exchange | |||||||||
| g | b/f | g | b/f | ring | dic | qr | tr | dmin | |||
| HepG2 | |||||||||||
| IC | 4 | 1 | 3 | - | - | - | - | - | - | 1 | 4 |
| SC | 3 | 1 | 3 | - | - | - | - | - | - | - | 3 |
| HF 0.75 | 2 | 1 | 2 | - | - | - | - | - | - | - | 2 |
| HF 1.5 | 2 | - | 2 | - | - | - | - | - | - | - | 2 |
| PC B(a)P 1.7 | 35 *** | - | 33 | 1 | 5 | - | 1 | - | - | - | 39 *** |
| HF 0.75 + B(a)P 1.7 | 8 ### | 1 | 7 | - | 1 | - | - | - | - | - | 8 ### |
| HF 1.5 + B(a)P 1.7 | 8 ### | 1 | 8 | - | - | - | 1 | - | - | - | 9 ### |
IC—intact control; SC—solvent control (DMSO); HF—hyperforin; PC B(a)P—positive control (benzo(a)pyrene); CA—chromosome aberrations; g—gap; b/f—break and/or fragment; dic—dicentric; qr—quadriradial; tr—triradial. Comparison with solvent control (SC): *** p < 0.001. Comparison with positive control (PC): ###p < 0.001.